This page contains links to our standardized lab protocols (as we write them). The majority are derived from other common protocols (references), and are tweaked a little for our lab environment. This page is intended for our internal reference, and makes no claim to be suitable for people outside our lab. This page is under constant revision and expansion, so check back periodically.
Molecular Biology Protocols:
E. coli Protocols:
Preparation of Electro-Competent Bacteria
Transformation of Bacteria by Electroporation
Heat Shock Transformation of Frozen, Chemically Competent E. coli
Agrobacterium Protocols:
Yeast Protocols:
Rapid isolation of yeast genomic DNA
Yeast Transformation (Lithium Acetate/PEG/ssDNA method) updated 11/15/13
Plant Protocols:
Floral-Dip Protocol for Arabidopsis Transformation
Hydroponic Growth of Arabidopsis thaliana
Collecting phloem sap exudations with EDTA solutions
Photosynthetic labeling with [14C]-CO2 for metabolism and transport studies
| Attachment | Size |
|---|---|
 Protocol for 14C HCO3 labeling.doc | 33 KB |