LSB 422
Lab: LIFE B470
Undergraduate students, graduate students, and postdocs are highly encouraged to contact Dr. Horn by email to inquire about openings in various projects aiming to study biochemical pathways for producing high value bioproducts, abiotic stress, membrane biology, and/or other topics that may be of mutual interest.
Degrees:
1. Identifying and Characterizing Enzymes in Plant Metabolism and Bioproduct Production:
The plant kingdom produces an astonishing number of chemically and functionally diverse metabolites that contribute to a plant's survival, growth and development, defense, and evolutionary selection. Identifying and characterizing all the genes, and their encoded proteins, that produce these metabolites and modulate their levels in vivo is a complex task that requires various techniques ranging from genetics to biochemistry to computational biology to omics and beyond. Our research focuses on identifying and characterizing proteins that produce high-value plant lipids (or oils) which are essential to plant growth and development, human health and nutrition (e.g., omega-3 fatty acids), and industrial feedstocks (e.g., biofuels, lubricants). This work targets biochemical pathways ranging from model plants (e.g., Arabidopsis thaliana) to crops important to the Texas economy (e.g., cotton) to non-domesticated plant systems (e.g., Sterculia foetida). Students and personnel working on these structure-function projects will primarily learn and use genetic engineering, molecular biology, biochemical, and structural biology techniques.
2. Elucidating the Structural and Functional Role of Plant Thiols:
Cysteines (Cys) are chemically reactive amino acids containing sulfur that play diverse roles in plant biology. Recent proteomics investigations in the model plant Arabidopsis thaliana have revealed the presence of thiol post-translational modifications (PTMs) in several Cys residues. These PTMs are presumed to impact protein structure and function, yet mechanistic data regarding the specific Cys susceptible to modification and their biochemical relevance remain limited. To help address these limitations, we are characterizing the structure and functional roles of Cys in enzymes susceptible to thiol PTMs. Given the thiol proteome also changes in response to abiotic stress conditions (e.g., high temperature, drought, etc.) understanding these processes at the basic science level will enable translational applications that contribute to enhanced plant resilience. Students and personnel working on these projects primarily learn and use biochemistry, plant physiology, and genetics techniques.
1. Cai Y, Horn P (2025). Packaging "Vegetable Oils": Insights into Plant Lipid Droplet Proteins. Plant Physiology Special Issue in Plant Lipids: Signaling and Metabolism. Issue Cover. doi/10.1093/plphys/kiae533/7905744
2. Johnston CR, Horn PJ, Alonso AP (2024). First draft reference genome and annotation of the alternative oil species Physaria fendleri. G3: Genes, Genomes, Genetics. 14, jkae114. doi.org/10.1093/g3journal/jkae114
3. Cannon AE, Horn PJ (2024). The Molecular Frequency, Conservation and Role of Reactive Cysteines in Plant Lipid Metabolism. Plant and Cell Physiology- Special Issue in Plant and Algal Lipids. pcad163 doi.org/10.1093/pcp/pcad163
4. Horn PJ, Chapman KD (2023). Imaging plants in situ. Journal of Experimental Botany- Special Issue in Plant Metabolism. erad423 doi.org/10.1093/jxb/erad423
5. Borisjuk L, Horn P, Chapman, K, Jakob PM, Gündel A, Rolletschek H (2023). Seeing plants as never before. New Phytologist Tansley Review 238, 1775-1794. doi/10.1111/nph.18871
1. Ayre, B.G. (PI), McGarry, R.C. (Co-PI), Macias, V.M. (Co-PI), Horn, P. (Co-PI), Shah, J. (Co-PI), "A Laser Microdissection System to Enhance Agricultural and Food Research in the North Texas and Southern Oklahoma Region," sponsored by USDA NIFA Equipment Grants Program, Federal, $341019. (2023 - 2027).
2. Horn, P. (PI), Cai, Y. (Co-PI), "Next-Generation Plant Lipid Droplets: Tailoring Organelle Function for Diverse Bioproducts," sponsored by BioDiscovery Institute, University of North Texas, $20000. (2025 - 2026).
3. Horn, P. (PI), Chapman, K. (Co-PI), "Elucidating Cyclic Fatty Acid Biosynthesis and Compartmentalization to Improve Cottonseed Value," sponsored by USDA-NIFA, Federal, $294000. (2022 - 2026).
Biochemistry I (BIOC 4540/5540), Cell Biology (BIOL 3510), Metabolic Engineering (BIOC 5680)