Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages | Department of Biological Sciences

COVID-19 updates—In an effort to keep everyone healthy, UNT's on-campus operations are closed until further notice. We're serving students remotely. Please stay connected. Stay up to date on UNT’s response to COVID-19 (Coronavirus).

Have you registered for fall classes yet? While COVID-19 has created some uncertainty for us all, UNT is committed to helping the Mean Green family turn dreams into reality. Let's get through this together!

Register for classes on my.unt.edu
Not a UNT student yet? Apply to UNT
Having trouble registering? Get help from an advisor

January 8, 2018

Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages


The vast bacteriophage population harbors an immense reservoir of genetic information. Almost 2000 phage genomes have been sequenced from phages infecting hosts in the phylum Actinobacteria, and analysis of these genomes reveals substantial diversity, pervasive mosaicism, and novel mechanisms for phage replication and lysogeny. Here, we describe the isolation and genomic characterization of 46 phages from environmental samples at various geographic locations in the U.S. infecting a single Arthrobacter sp. strain. These phages include representatives of all three virion morphologies, and Jasmine is the first sequenced podovirus of an actinobacterial host. The phages also span considerable sequence diversity, and can be grouped into 10 clusters according to their nucleotide diversity, and two singletons each with no close relatives. However, the clusters/singletons appear to be genomically well separated from each other, and relatively few genes are shared between clusters. Genome size varies from among the smallest of siphoviral phages (15,319 bp) to over 70 kbp, and G+C contents range from 45-68%, compared to 63.4% for the host genome. Although temperate phages are common among other actinobacterial hosts, these Arthrobacter phages are primarily lytic, and only the singleton Galaxy is likely temperate.

The complete Research Article