This page contains links to our standardized lab protocols (as we write them). The majority are derived from other common protocols (references), and are tweaked a little for our lab environment. This page is intended for our internal reference, and makes no claim to be suitable for people outside our lab. This page is under constant revision and expansion, so check back periodically.

Molecular Biology Protocols:

E. coli Protocols:

Preparation of Electro-Competent Bacteria

Transformation of Bacteria by Electroporation

Heat Shock Transformation of Frozen, Chemically Competent E. coli

Alkaline Lysis Mini-Preps

Agrobacterium Protocols:

Yeast Protocols:

Rapid isolation of yeast genomic DNA

Yeast Transformation (Lithium Acetate/PEG/ssDNA method) updated 11/15/13

Plant Protocols:

Floral-Dip Protocol for Arabidopsis Transformation

Hydroponic Growth of Arabidopsis thaliana

Collecting phloem sap exudations with EDTA solutions

Photosynthetic labeling with [14C]-CO2 for metabolism and transport studies

Labeling leaf disks with [14C]-Suc