Spinning Disk Confocal Microscope

A set of images of cells undergoing mitosis: prophase, metaphase, anaphase, teleophase, and cytokineses.

Sample prepared by Hemangi Patil, 2009

The fluorophores that were used are: blue: Hoechst, Green: Fluorescein, and Red: Texas Red. The fluorophores were excited with 405, 488 and 568 nm lasers. The images were collected with a Hamamatsu camera set for a 64 gain for the blue channel and a 128 gain in the green and red channels. The emission light was collected with an 100x magnification oil emmersion objective.