P0 ICR mouse brain with two astrocytes perpendicular to a blood vessel.
Sample prepared by Kimberly Spurgeon December 2006.

The Pericentrin flourophore was excited with a  405 nm laser and the emission was collected with a 450/35 nm band pass filter and is colored blue.
The GFAP flourophore was excited with 488 nm laser and the emission was collected with a 520/35 nm band pass filter and is colored green.
The AC3 flourophore was excited with 568 nm laser and the emission was collected with a 593/45 nm band pass filter and is colored red. 
40x magnification.