This is a set of images of cells undergoing mitosis: prophase, metaphase, anaphase, teleophase, and cytokineses.

The fluorophores that were used are: blue: Hoechst, Green: Fluorescein, and Red: Texas Red.  The fluorophores were excited with 405, 488 and 568 nm lasers.  The images were collected with a Hamamatsu camera set for a 64 gain for the blue channel and a 128 gain in the green and red channels.  The emission light was collected with an 100 times magnification oil emmersion objective.  
Sample prepared by Hemangi Patil, January 2009.